MIB-30806 Applied Molecular Microbiology


Credits 6.00

Teaching methodContact hours
Problem-based learning12
Course coordinator(s)prof. dr. J van der Oost
Lecturer(s)prof. dr. J van der Oost
dr. ir. LH de Graaff
prof. dr. M Kleerebezem
dr. RA Weusthuis
dr. SJJ Brouns
Examiner(s)prof. dr. J van der Oost
dr. ir. LH de Graaff
prof. dr. M Kleerebezem

Language of instruction:


Assumed knowledge on:

Microbiology & Biochemistry, Microbial Physiology, Biotechnology 1, Cell physiology & Gentechnology

Continuation courses:

Thesis/Minor/Stage BIC, CBI, GEN, MOB, MIB, VIR


For thousands of years the natural bio-converting capacity of many microorganisms is being exploited by man in a variety of 'classical' biotechnology applications. Over the last 50 years, a dramatic gain of insight in molecular genetics of microorganisms has resulted in the development of the 'modern' biotechnology: the engineering of microorganisms and their enzymes to further enhance the efficiency of specific bio-conversions. The goal of the course Applied Molecular Microbiology is to provide insight in the molecular details of a variety of biotechnological processes, and to give an overview of the currently available set of methods that are being used to optimize specific bioconversions.
The book 'Microbiology' (Prescott, Harley, Klein - McGraw-Hill - 6th edition 2005) describes most of the basic knowledge, and it is strongly recommended to use this book during the course. Additional information will be described in the reader Applied Molecular Microbiology. Apart from lectures from the three lecturers and several guest lecturers, the students will participate in 'case studies' in which specific problems are addressed, either in small groups or individually (coordinated by Leo de Graaff).
Participation: Maximum number of students is 72 (register via Eduweb)

Learning outcomes:

After this course the students is expected to be able to:
- understand the principles of relevant classical and modern molecular genetics tools;
- select appropriate engineering methods to address specific biotechnology problems;
- analyze molecular genetics reports in the biotechnology literature, and prepare a concise report and/or presentation on a given case.


The students work in small groups on a number of cases related to the different theme's of the course.


Written examination (75%) and reports of the cases (25%).


Reader Applied Molecular Microbiology and 'Microbiology' (Prescott et al. 7th ed.).

Compulsory for: MBTBiotechnologyMScA: Cellular/Molecular Biotechnology2MO
Restricted Optional for: MBTBiotechnologyMScC: Medical Biotechnology2MO
MBTBiotechnologyMScB: Food Biotechnology2MO
MMLMolecular Life SciencesMScB: Biological Chemistry2MO