BIP-32803 Biophysical Imaging

Course

Credits 3.00

Language of instruction:

English

Assumed knowledge on:

Analitical Chemistry or Spectroscopy.

Contents:

Imaging techniques play an important role in biophysical research. In this course the principles of spectroscopic microscopy [confocal laser scanning microscopy, fluorescence (life time) and NMR imaging (MRI), ultra-microscopy (atomic force and scanning electron microscopy) will be presented. In addition, principles of image processing and analysis, manipulation and interpretation of two- and three dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.

Learning outcomes:

Upon following this course the student will have gained knowledge of the available techniques for imaging of biological systems, enabling the proper choice and use of the techniques in practical biophysical research.

Activities:

- study syllabus;
- solve exercises, do practical experiments;
- write a report;
- use ICT for analysis of results and present results.

Examination:

This course will be judged based on active participation, understanding, presentation, results and written reports as follows:
- execution of problems and experiments (10%);
- understanding of the methods (20%);
- execution of the reports (50%);
- presentation of the results and conclusions of the projects (20%).
In these elements the MRI part counts for 50%, the FLIM/CLSM and the AFM parts each for 25%.

Literature:

Syllabus and manual for practical's will be handed over during the course.