MOB-20306 Gene Technology

Course

Credits 6.00

Language of instruction:

English

Assumed knowledge on:

BIC-10306 Practicum Biologische Chemie and BIC-20306 Cellphysiology and Genetics or BIC-23103 Cellphysiology

Continuation courses:

MOB-31303 Molecular Development; MOB-30306 Control of cellular processes and cell differentiation; MOB-30806 Regulation of plant development

Contents:

Recombinant DNA technology has brought about a revolution in our understanding of molecular processes in living organisms. To date, there is no field in experimental biology that has remained untouched by the potential of isolating, analyzing and manipulating genes and organisms. Thus, gene technology provides essential tools in both fundamental and applied medical, industrial, agricultural, environmental and biological research.
In this course we will focus on four major topics, including (i) production of transgenic mammals, (ii) disrupting gene functions using knockouts and RNAi, (iii) transgenic mice as human model systems and (iv) production of transgenic crops. In addition to conventional lectures that will outline the major procedures, applications and some research papers, students will be challenged by virtual (computer based modules) experimental designs on the generation and characterization of transgenic animals and plants.
The practical course involves a set of basic biomolecular techniques, including construction of a small genomic DNA library, site-directed mutagenesis, PCR, and hybridisation analysis. These experiments will first be carried in a virtual environment (the simple cloning lab).
Due to a limited number of places available in the practical part of the course, students are encouraged to register in time.

Aims:

Understand methods currently used to clone, analyze and manipulate genes and to introduce foreign genes into animals and plants. To obtain experience with experimental strategies and with interpretation of experimental data involving gene technology. To be able to apply basic recombinant DNA methods, interpret the results of the experiment, and incorporate the results in a written report. To be able to apply basic regulations when working with genetically modified micro-organisms.

Activities:

Lectures; computer-based designs and analysis of experiments involving cloning, and construction of transgenic animals and plants; laboratory experiments, writing a report of the practical course.

Examination:

The final mark will be based on a written examination on open questions, laboratory skills and on the quality of report of the practical part.