ORC-11806 Analytical Methods in Organic Chemistry

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Studiepunten 6.00

Language of instruction:

English and Dutch

Assumed knowledge on:

ORC-12803 Bio-organic Chemistry I and ORC-12903 Bio-organic Chemistry II

Contents:

This course introduces the student:
(1) to both theory and practical application of chromatographic analyses and separation techniques, and understanding and using basic isolation techniques for components of various chemical classes. Attention is given to the analysis of e.g. preservatives sugars and pollutants using a variety of techniques (e.g. TLC, HPLC, GC, and capillary electrophoresis), and to test the purity of samples obtained with these techniques; (2) to both theory and practical applications of spectroscopic methods aiming towards the structural analysis of organic compounds, e.g. as obtained via the methods described under (1). The combination of UV/VIS, IR & NMR spectroscopy and mass spectrometry (MS) is discussed and used. During the practical work these methods will be applied towards the elucidation of the structure of unknown compounds.
(3) special emphasis is also given to combining (1) and (2): the application of GC-MS and LC-MS including theory, scope and limitations, and practical usage in the analysis of samples from sports doping and food forensics.

Learning outcomes:

After successful completion of this course students are expected to be able to:
- reproduce chromatographic and spectroscopic theory and use this to solve problems; - apply various isolation and chromatographic techniques to separate compounds; - appraise published analytical procedures and explain the logic behind them; - apply spectroscopic and sample preparation techniques to obtain spectra from compounds; - interpret recorded spectra and propose a chemical structure based on those data; - prepare a written report that summarises collected data in a clear fashion.

Activities:

Attend lectures, problem solving meetings and case study, study of the book and reader, doing practical experiments and simulation experiments, report writing.

Examination:

Written exam with open questions. Mark for practical work is based on practical level, input during practical classes and reports.

Literature:

J.W. Robinson, E.M. Skelly Frame, G.M. Frame II, Undergraduate Instrumental Analysis, 6th ed., Marcel Dekker, New York. Course Manual ORC-11806.