MIB-10306 Microbiology & Biochemistry


Studiepunten 6.00

Course coordinator(s)dr. SWM Kengen
dr. ir. D Sipkema
Lecturer(s)dr. SWM Kengen
dr. ir. CPM van Mierlo
dr. ir. D Sipkema
I Rabbers
MSc PGB Puylaert
Examiner(s)dr. ir. CPM van Mierlo
dr. SWM Kengen
dr. ir. D Sipkema

Language of instruction:


Mandatory knowledge:

ZSS06100 Laboratory Safety

Assumed knowledge on:

Microbiological knowledge is no prerequisite. It is an advantage when you have attended the Cell biology course (CBI10306) and have basic knowledge of (in)organic and physical chemistry. In case of deficiencies contact the lecturers.

Continuation courses:

Microbial Physiology MIB20306


Note: This course can not be combined in an individual programme with MIB-11306 Microbiology and Biochemistry for Nutrition and Health.

Genetic, physiological, morphological and ecological aspects of prokaryotes and primitive eukaryotes are addressed. The omnipresence and diversity of microorganisms is the central item.

Learning outcomes:

After successful completion of this course students are expected to be able to:
- explain the importance of the large diversity of microorganisms for development and sustainment of life on earth;
- explain that structure and function of enzymes are a link between chemistry and the living cell, and that biochemical unity underlies biological diversity;
- explain the following basic principles of metabolism of (micro)organisms: manipulation of free energy by enzymes to direct metabolism, importance of oxidation-reduction reactions for extracting energy from organic and inorganic substrates, glycolysis, citric acid cycle and respiratory chain;
- compare the basal metabolism with glucose as a model substrate in (micro)organisms in the presence and absence of external electron acceptors;
- summarize a number of microbial interactions in specific ecosystems;
- exemplify the influence of environmental factors on microbial activity in the cycling of nutrients, like carbon, nitrogen, sulfur and iron;
- practice a set of basic microbial experiments with emphasis on aseptic techniques for transfer and handling of microorganisms;
- enrich and isolate microorganisms under defined environmental conditions.


- attend lectures that are supported by 2 textbooks;
- perform several laboratory experiments;
- assess acquired practical knowledge by answering questions, compose a lab journal and give a short presentation.


The final mark is based on:
One digital exam composed of 50 multiple choice questions (covering the Microbiology lectures and the practical) and about 7 open and 3 multiple choice questions (covering the Biochemistry lectures). To pass the exam the marks for the MIB- and BIC-parts should each be at least 5.00 and the overall average mark (MIB ¾ + BIC ¼) should be at least 5.50.


Textbooks are:
Michael T. Madigan [et.al]. (2018). Brock Biology of Microorganisms. 15th Global edition. Pearson, ISBN 13: 9781292235103.
Jeremy M. Berg; John L. Tymoczko; Lubert Stryer. (2019). Biochemistry. 9th edition. WH Freeman; ISBN: 1319114652
Textbook and practical course handbook are available at the WUR-shop in the Forum.

Verplicht voor: BBIBiologyBSc2AF
BMLMolecular Life SciencesBSc3AF+4AF
Keuze voor: BATBiosystems EngineeringBSc2AF, 3AF+4AF