Language of instruction:
ZSS06100 Laboratory Safety
Assumed knowledge on:
FHM-20306 Food Microbiology.
FHM-30306 Food Safety Risk Assessment or thesis FHM.
This course aims to provide insight in the ecology and physiology of food and Gastro-intestinal-tract microbes. Ecology and physiology of food-associated spoilage microbes and food-borne human pathogens is discussed in relation to behaviour in the food chain with a focus on performance in food processing environments. Microbial stress response in (minimal) processing is discussed including mechanisms of action of (novel) processing strategies, their cellular targets and corresponding microbial defence systems that support survival and thus could affect efficacies of processing technologies and preservation regimes. Ecology in the food chain addresses classical and novel microbial detection and identification methods including whole genome sequencing approaches and their application in practice. Design and implementation of sampling strategies and plans are discussed and linked to other tools for the production of safe foods including food safety objectives (FSOs) and risk analysis. The composition and putative functions of prebiotics and probiotics is explained, together with an overview of the ecology and functionality of GI tract microbiota. In addition, during the practical course experience with a range of techniques will be obtained to collect microbiological data from (model) foods and faecal samples, to perform data analysis, to present results, and to prepare reports on the food microbiology experiments.
After successful completion of this course students are expected to be able to:
- discuss the contribution of viruses, bacteria and fungi to food-borne diseases;
- illustrate novel minimal processing methods used in food industries;
- compare effects of preservation factors and techniques on microbes;
- explain survival strategies employed by bacteria;
- dissect key parameters in biofilm development;
- select and categorize methods for detection and identification of microbes for assessment of food quality and safety;
- design microbiological sampling plans to control the microbiological quality and safety of food products;
- explain FSOs and risk analysis for the production of safe Foods;
- explain the ecology and functionality of GI tract microbes, and effects of pre- and probiotics;
- select and apply methods to characterize the microbial composition and activity in the GI tract and faecal samples;
- collect microbiological data, perform data analysis, present results, and prepare reports on microbiology experiments.
The activities consist of:
- formal lectures;
- laboratory classes. The practical course will be held in ML2 labs and corresponding safety regulations prevail.
- written and oral presentations.
Final grading is based on a written exam (70%) with open questions and the practicals (30%). For both elements a minimum pass mark of 5.5 is required.
The grade for the practicals is valid for 3 years.
All materials provided in Brightspace.
|Verplicht voor:||MFS||Food Safety||MSc||A: Spec. A - Applied Food Safety||3WD|
|Keuze voor:||MBT||Biotechnology||MSc||B: Spec. B - Food Biotechnology||3WD|
|MFT||Food Technology||MSc||C: Spec. C - Food Fermentation and Biotechnology||3WD|
|MFS||Food Safety||MSc||C: Spec. C - Supply Chain Safety||3WD|