BIP-32803 Biophysical Imaging


Studiepunten 3.00

Course coordinator(s)dr. JC Hohlbein
Lecturer(s)dr. LH Beun
dr. JC Hohlbein
C Terenzi PhD
dr. AN Bader
Examiner(s)dr. JC Hohlbein

Language of instruction:


Assumed knowledge on:

Analitical Chemistry or Spectroscopy.


Note: This course has a maximum number of participants (and has on top of that reserved places for new MSc students). The deadline for registration is one week earlier than usual. See Academic Year ( -> Registration for Courses.
Imaging techniques play an important role in many scientific disciplines and especially in biophysical research. In this intensive practical course, students will learn the principles of various imaging techniques based on spectroscopic microscopy [confocal laser scanning microscopy, fluorescence life time imaging and nuclear magnetic resonance imaging] and high-resolution microscopy (atomic force and single-molecule). In addition, principles of image processing, data analysis, and interpretation of multi-dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.

Learning outcomes:

After successful completion of this course students are expected to be able to:
•    explain the basic principles of four spectroscopic methods for imaging of biological or food-related systems;
     - Atomic Force Microscopy (AFM);
     - Laser Scanning Microscopy (LSM);
     - Magnetic Resonance Imaging (MRI);
     -  Single-Molecule Localisation Microscopy (SMLM).
•    apply these techniques knowing their particular limitations and strengths (achievable spatial resolution, contrast, information content and possible applications);
•    understand and apply suitable image and data processing techniques;
•    interpret the results in terms of (spatial and dynamic) information.


- understanding of the methods (AFM, LSM, MRI, SMLM);
- execution of experiments (AFM, LSM, MRI, SMLM);
- active participation (asking questions, reading papers, preparing peer feedback);
- preparing of the reports or a poster;
- preparing oral presentation.


This course will be judged based on active participation, understanding, presentation, results and written reports. The MRI and SMLM parts counts for 30 % each, the LSM and AFM parts each for 20%.


Syllabus and manual for practicals will be available before the start of the course.

Keuze voor: MBTBiotechnologyMScD: Spec. D - Process Technology4MO
MMLMolecular Life SciencesMSc4MO