PPH-30306 Plant Cell and Tissue Culture


Credits 6.00

Teaching methodContact hours
Practical intensively supervised70
Course coordinator(s)dr. D Vreugdenhil
Lecturer(s)dr. D Vreugdenhil
dr. ing. NCA de Ruijter
prof. dr. HJ Bouwmeester
dr. ir. MJ Ketelaar
Examiner(s)dr. D Vreugdenhil
prof. dr. ir. MGM Aarts
prof. dr. HJ Bouwmeester
prof. dr. ME Janson

Language of instruction:


Assumed knowledge on:

PCB-10306; PPH-20306; GEN-10306

Continuation courses:

MSc thesis PPH, PCB, GEN


The aim of this course is to provide a thorough understanding of theoretical and practical aspects of plant cell and tissue culture.
Topics include: plant regeneration, genetic modification, cellular and physiological aspects of differentiation, somatic embryogenesis, secondary metabolism, hormonal regulation of differentiation.

Learning outcomes:

After this course the students are able to:
- explain and discuss the general theoretical backgrounds of plant cell and tissue culture, comprising regeneration, plant genetic modification, cellular and physiological aspects of differentiation of plant tissue cultures (including hormonal aspects), somatic embryogenesis, secondary metabolism;
- find, read, understand and integrate literature in the field of plant cell and tissue culture, comprising the aspects mentioned under the previous point;
- devise, carry out and interpret experiments in plant cell and tissue culture, comprising a.o. handling various forms of plant tissue culture (including somatic embryogenesis and anther/microspore cultures), induction of secondary metabolite pathways, Agrobacterium transformation and induction of mutants, using general prescriptions;
- devise, carry out, interpret and present a non-standard experiment in the field of plant cell and tissue culture, based on own literature search and own ideas.


Theoretical aspects will be taught during lectures and by studying and discussing recent research papers. A large part of the course will be devoted to practical work. Besides 'standard' experiments, students will devise, set up and carry out their own new experiment.


Written examination and reports of the experiments.


At the start of the course, a syllabus will be supplied.

Restricted Optional for: MBIBiologyMScA: Cell Biology5AF
MBIBiologyMScA: Cell Biology5AF
MBIBiologyMScB: Plant Biology5AF
MBIBiologyMScB: Plant Biology5AF
MBTBiotechnologyMScA: Cellular/Molecular Biotechnology5AF
MPSPlant SciencesMScB: Greenhouse Horticulture5AF
MPBPlant BiotechnologyMScA: Functional Plant Genomics5AF
MPBPlant BiotechnologyMScB: Plants for Human and Animal Health5AF