|Teaching method||Contact hours|
|Practical extensively supervised||44|
|Practical intensively supervised||36|
|Course coordinator(s)||dr. ir. JE Wellink|
|Lecturer(s)||dr. ir. JE Wellink|
|ir. JWG Verver|
|dr. ir. R Geurts|
|Examiner(s)||dr. HGJM Franssen|
|dr. ir. R Geurts|
Language of instruction:
Assumed knowledge on:
BIC-10306 Practical Biological Chemistry and BIC-20306 Cell Physiology and Genetics or GEN-11806 Fundamentals of Genetics and Molecular Biology
MOB-31303 Molecular Development; MOB-30306 Control of cellular processes and cell differentiation; MOB-30806 Regulation of plant development
Recombinant DNA technology has brought about a revolution in our understanding of molecular processes in living organisms. To date, there is no field in experimental biology that has remained untouched by the potential of isolating, analyzing and manipulating genes and organisms. Thus, gene technology provides essential tools in both fundamental and applied medical, industrial, agricultural, environmental and biological research.
In this course we will focus on four major topics, including (i) production of transgenic mammals, (ii) disrupting gene functions using knockouts and RNAi, (iii) transgenic mice as human model systems and (iv) production of transgenic crops. In addition to conventional lectures that will outline the major procedures, applications and some research papers, students will be challenged by virtual (computer based modules) experimental designs on the generation and characterization of transgenic animals and plants. The practical course involves a set of basic biomolecular techniques, including construction of a small genomic DNA library, site-directed mutagenesis, PCR, and hybridisation analysis. These experiments will first be carried out in a virtual environment (The Simple Cloning Lab).
Due to a limited number of places available in the practical part of the course, students are encouraged to register in time. Students for with this course is compulsory or restricted optional take precedence.
At the end of the course, the student is expected to be able:
- to understand methods currently used to clone, analyze and manipulate genes and to introduce foreign genes into animals and plants;
- to design experimental strategies and interpret experimental data involving gene technology;
- to apply basic recombinant DNA techniques, to interpret the results of these experiments involving these techniques, and to incorporate the results in a written report;
- to apply basic regulations when working with genetically modified micro-organisms.
Lectures; computer-based designs and analysis of experiments involving cloning, and construction of transgenic animals and plants; laboratory experiments, writing a report of the practical course.
The final mark will be based on a written examination with open and multiple choice questions, laboratory skills and on the quality of report of the practical part.
Reader, and manual for the practical part.
|Compulsory for:||BBI||Biology||BSc||A: Cell and Molecular Biology||1AF|
|BML||Molecular Life Sciences||BSc||6WD|
|Restricted Optional for:||MPS||Plant Sciences||MSc||E: Plant Pathology and Entomology||1AF, 6WD|
|MPS||Plant Sciences||MSc||D: Plant Breeding and Genetic Resources||1AF, 6WD|
|MPB||Plant Biotechnology||MSc||1AF, 6WD|
|MNH||Nutrition and Health||MSc||C: Molecular Nutrition and Toxicology||1AF, 6|
|Restricted Optional for:||WUBIT||BSc Minor Biotechnology||6WD|