MIB-10306 Microbiology & Biochemistry

Course

Credits 6.00

Language of instruction:

Dutch

Contents:

Genetic, physiological, morphological and ecological aspects of procaryotes and primitive eucaryotes are addressed. Diversity of microorganisms is the central item.

Learning outcomes:

At the end of the course the student is expected to be able to demonstrate a detailed understanding of:
- importance of microorganisms for development of and to sustain life on earth;
- biochemical unity that underlies biological diversity;
- function of enzymes as a link between chemistry and the living cell;
- large diversity of microorganisms (including their metabolism);
- importance of free energy, enzymatic reactions and oxidation-reduction reactions;
- basal metabolism of glucose: glycolysis, citric acid cycle and respiratory chain;
- genotypic and phenotypic changes of bacteria (including gene-transfer);
- microbial cycling of nutrients (incl. carbon, nitrogen, sulfur and metals);
- microbial interactions with living and non-living environments;
- influence of environmental factors on microbial activity. The student is expected to be able to:
- properly use aseptic techniques for transfer and handling of microorganisms;
- properly use a phase-contrast and a bright-field light microscope for microscopic examination;
- enrich and isolate microorganisms under defined environmental conditions;
- use standard microbiology laboratory equipment correctly;
- perform and understand a number of simple microbial experiments.

Activities:

Attend lectures that are supported by 2 textbooks. Perform several laboratory experiments, write a laboratory report and give a small presentation.

Examination:

The final mark is based on a written exam and on the outcome of the laboratory experiments (practical work + laboratory report)

Literature:

Textbooks are "Biology of Microorganisms" (Brock 12th ed.) and "Biochemistry" (Berg, Tymoczko, Stryer 6th ed.). Textbook and practical course handbook are available at WUR shop, Forum.