BIP-32803 Biophysical Imaging

Code last year: (BIP-22303)


Credits 3.00

Teaching methodContact hours
Practical extensively supervised24
Practical intensively supervised40
Course coordinator(s)dr. H Van As
Lecturer(s)dr. H Van As
ir. FJ Vergeldt
dr. ing. JW Borst
dr. ir. JM Kleijn
AH Westphal
Examiner(s)dr. H Van As

Language of instruction:


Assumed knowledge on:

Analitical Chemistry or Spectroscopy or Biological Spectroscopy


Imaging techniques play an important role in biophysical research. In this course the principles of spectroscopic microscopy [confocal laser scanning microscopy, fluorescence (life time) and NMR imaging (MRI), ultra-microscopy (atomic force and scanning electron microscopy) and photothermal spectroscopy (photo-acoustic and opto-thermal window spectroscopy) will be presented. In addition, principles of image processing and analysis, manipulation and interpretation of two- and three dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.

Learning outcomes:

Upon following this course the student will have gained knowledge of the available techniques for imaging of biological systems, enabling the proper choice and use of the techniques in practical biophysical research.


Study syllabus; solve exercises, do practical experiments; write a report; use ICT for analysis of results, present results.


Evaluation of study results will be based on presentation, results and written reports.


Syllabus and manual for practicals will be handed over during the course.

Restricted Optional for: MBTBiotechnologyMScC: Medical Biotechnology4MO
MBTBiotechnologyMScD: Process Technology4MO
MMLMolecular Life SciencesMSc4MO
MMLMolecular Life SciencesMScA: Biomedical Research4MO