ORC-11806 Analytical Methods in Organic Chemistry

Course

Credits 6.00

Teaching methodContact hours
Lecture12
Tutorial24
Practical70
Course coordinator(s)dr. ir. LCPM de Smet
Lecturer(s)dr. ir. LCPM de Smet
dr. TA van Beek
prof. dr. MWF Nielen
SP Pujari
dr. ir. HM Willemen
dr. GIJ Salentijn
Examiner(s)dr. TA van Beek
dr. ir. LCPM de Smet
dr. GIJ Salentijn

Language of instruction:

NL and/or EN

Mandatory knowledge:

ZSS06100 Laboratory Safety


Assumed knowledge on:

ORC-12803 Organic Chemistry 1 and ORC-12903 Organic Chemistry 2

Contents:

Note: This course has a maximum number of participants. The deadline for registration is one week earlier than usual. See Academic Year (http://www.wur.nl/en/Education-Programmes/Current-Students/Agenda-Calendar-Academic-Year.htm) => Registration for Courses.
This course introduces the student:
(1) To both theory and practical application of chromatographic analyses and separation techniques, and understanding and using basic isolation techniques for components of various chemical classes. Attention is given to the qualitative and quantitative analysis of, e.g., preservatives, drugs, sugars and pollutants using a variety of techniques (e.g., TLC, HPLC, GC, capillary electrophoresis (CE) and ELISA).(2) To both theory and practical applications of spectroscopic methods aiming towards the structural analysis of organic compounds, including proteins, e.g., as obtained via the methods described under (1). The combination of UV/VIS, IR & NMR spectroscopy and mass spectrometry (MS) is discussed and used. During the practical work these methods will be applied towards the elucidation of the structure of unknown compounds.
(3) Special emphasis is also given to combining (1) and (2): the application of GC-MS and LC-MS including theory, scope and limitations, and practical usage in the analysis of food contaminants.

Learning outcomes:

After successful completion of this course students are expected to be able to:
• Understand chromatographic theory and use this to solve problems;
• Understand spectroscopic theory and use this to solve problems;
• Apply (theoretically and practically) various extraction, sample clean-up, and chromatographic techniques to separate compounds;
• Appraise published analytical procedures and explain the logic behind them;
• Apply (theoretically) sample preparation and spectroscopic techniques to obtain spectra from compounds;
• Interpret recorded spectra and propose a chemical structure based on those data;
• Prepare a written chromatography report on the chromatography practical sessions;
• Prepare a written spectroscopy report that summarizes collected data in a clear fashion.

Activities:

- follow archived online lectures;
- participate in online exercises classes (tutorials) and follow an archived case study (lecture);
- study of the book and reader;
- doing on-campus practical chromatography experiments (5  4 hours) and on-line simulation experiments/assignments;
- participate in on-campus PI sessions on spectroscopy (4  4 hours) and online PI sessions;
- report writing.

Examination:

- the final mark is determined by the written exam (60%);
- the practicals and reports chromatography (40%);
The minimum mark for each part is 5.00. The minimum average mark of the 2 parts to pass the course is 5.50. The written exam has open questions. Mark for practical work is based on practical level and input during practical classes and the two reports. The marks for the individual parts of this course will remain valid for 4 academic years.

Literature:

‘Organic Chemistry, 9th Edition, McMurry; ISBN 978-305-08048-5 and the reader ORC-11806 Analytical Methods in Organic Chemistry (a new version is published each year near the end of P5). Both are available at the WUR-shop.

ProgrammePhaseSpecializationPeriod
Compulsory for: BBTBiotechnologyBSc6WD
BMLMolecular Life SciencesBSc1AF
MinorPeriod
Restricted Optional for: WUCHMBSc Minor Chemical Sciences1AF