BIP-32803 Biophysical Imaging


Credits 3.00

Teaching methodContact hours
Course coordinator(s)dr. JC Hohlbein
Lecturer(s)C Terenzi PhD
dr. AN Bader
dr. JC Hohlbein
dr. LH Beun
Examiner(s)dr. JC Hohlbein

Language of instruction:


Assumed knowledge on:

Analitical Chemistry or Spectroscopy.


Note: This course has a maximum number of participants (and has on top of that reserved places for new MSc students). The deadline for registration is one week earlier than usual. See Academic Year ( -> Registration for Courses.
Imaging techniques play an important role in biophysical research. In this course the principles of spectroscopic microscopy [confocal laser scanning microscopy, fluorescence (life time) and NMR imaging (MRI), ultra-microscopy (atomic force microscopy) will be presented. In addition, principles of image processing and analysis, manipulation and interpretation of two- and three dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.

Learning outcomes:

After successful completion of this course students are expected to be able to:
- understand the available techniques for imaging of biological systems,
- make the proper choice and use of the techniques in practical biophysical research.


- study syllabus;
- solve exercises, do practical experiments;
- write a report;
- use ICT for analysis of results and present results.


This course will be judged based on active participation, understanding, presentation, results and written reports as follows:
- execution of problems and experiments (10%);
- understanding of the methods (20%);
- execution of the reports (50%);
- presentation of the results and conclusions of the projects (20%).
In these elements the MRI part counts for 50%, the FLIM/CLSM and the AFM parts each for 25%.


Syllabus and manual for practicals will be available before the start of the course.

Restricted Optional for: MBTBiotechnologyMScD: Process Technology4MO
MMLMolecular Life SciencesMSc4MO