BIP-32803 Biophysical Imaging

Course

Credits 3.00

Teaching methodContact hours
Practical64
Course coordinator(s)dr. JC Hohlbein
Lecturer(s)C Terenzi PhD
dr. AN Bader
dr. JC Hohlbein
dr. LH Beun
Examiner(s)dr. JC Hohlbein

Language of instruction:

English

Assumed knowledge on:

Analitical Chemistry or Spectroscopy.

Contents:

Note: This course has a maximum number of participants (and has on top of that reserved places for new MSc students). The deadline for registration is one week earlier than usual. See Academic Year (http://www.wur.nl/en/Education-Programmes/Current-Students/Agenda-Calendar-Academic-Year.htm) -> Registration for Courses.
Imaging techniques play an important role in biophysical research. In this course the principles of spectroscopic microscopy [confocal laser scanning microscopy, fluorescence (life time) and NMR imaging (MRI), ultra-microscopy (atomic force microscopy) will be presented. In addition, principles of image processing and analysis, manipulation and interpretation of two- and three dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.

Learning outcomes:

After successful completion of this course students are expected to be able to:
- understand the available techniques for imaging of biological systems,
- make the proper choice and use of the techniques in practical biophysical research.

Activities:

- study syllabus;
- solve exercises, do practical experiments;
- write a report;
- use ICT for analysis of results and present results.

Examination:

This course will be judged based on active participation, understanding, presentation, results and written reports as follows:
- execution of problems and experiments (10%);
- understanding of the methods (20%);
- execution of the reports (50%);
- presentation of the results and conclusions of the projects (20%).
In these elements the MRI part counts for 50%, the FLIM/CLSM and the AFM parts each for 25%.

Literature:

Syllabus and manual for practicals will be available before the start of the course.

ProgrammePhaseSpecializationPeriod
Restricted Optional for: MBTBiotechnologyMScD: Process Technology4MO
MMLMolecular Life SciencesMSc4MO