|Teaching method||Contact hours|
|Course coordinator(s)||dr. JC Hohlbein|
|Lecturer(s)||dr. JC Hohlbein|
|dr. AN Bader|
|dr. TE Kodger|
|dr. E Wientjes|
|C Terenzi PhD|
|Examiner(s)||dr. JC Hohlbein|
Language of instruction:
Assumed knowledge on:
Analitical Chemistry or Spectroscopy.
Note: This course has a maximum number of participants (and has on top of that reserved places for new MSc students). The deadline for registration is one week earlier than usual. See Academic Year (http://www.wur.nl/en/Education-Programmes/Current-Students/Agenda-Calendar-Academic-Year.htm) -> Registration for Courses.
Imaging techniques play an important role in biophysical research. In this course the principles of spectroscopic microscopy [confocal laser scanning microscopy, fluorescence (life time) and NMR imaging (MRI), ultra-microscopy (atomic force microscopy) will be presented. In addition, principles of image processing and analysis, manipulation and interpretation of two- and three dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.
After successful completion of this course students are expected to be able to:
- understand the available techniques for imaging of biological systems,
- make the proper choice and use of the techniques in practical biophysical research.
- study syllabus;
- solve exercises, do practical experiments;
- write a report;
- use ICT for analysis of results and present results.
This course will be judged based on active participation, understanding, presentation, results and written reports as follows:
- execution of problems and experiments (10%);
- understanding of the methods (20%);
- execution of the reports (50%);
- presentation of the results and conclusions of the projects (20%).
In these elements the MRI part counts for 50%, the FLIM/CLSM and the AFM parts each for 25%.
Syllabus and manual for practicals will be available before the start of the course.
|Restricted Optional for:||MBT||Biotechnology||MSc||D: Process Technology||4MO|
|MML||Molecular Life Sciences||MSc||4MO|