BIP-24306 Spectroscopy and Imaging


Credits 6.00

Teaching methodContact hours
Course coordinator(s)C Terenzi PhD
dr. E Wientjes
Lecturer(s)dr. ing. HB Albada
dr. AN Bader
CJAM Wolfs
ing. RBM Koehorst
dr. JC Hohlbein
KW Milc
M Mayar
C Terenzi PhD
S Yang
dr. E Wientjes
Examiner(s)C Terenzi PhD
dr. E Wientjes

Language of instruction:


Assumed knowledge on:

BIP-10303 Physics MLS, BIP-2AB06 Atomic and Molecular structure; ORC-11806 Analytical Methods in Organic Chemistry.

Continuation courses:

BNT-20806 Bio-Inorganic Chemistry; BIP-32306 Biophotonics and Photosynthesis; BIP-32803 Biophysical Imaging; BIP-31806 Advances in Magnetic Resonance.


This course deals with the physical background of various spectroscopic techniques and the use of these techniques in the analysis of the structure and dynamics of organic and biological compounds. The course will focus on optical spectroscopy (UV/VIS absorption, fluorescence and phosphorescence), magnetic resonance spectroscopy (NMR and ESR) and the use of these techniques for imaging (fluorescence microscopy and MRI). Tutorial's will complement lectures. Practical's and lab projects will provide training in the use of spectroscopic equipment, interpretation of spectroscopic data and image analysis.

Learning outcomes:

After successful completion of this course students are expected to be able to:
- understand relation between electronic structure and spectroscopic properties;
- understand the interaction of spins with magnetic fields;
- select and apply proper method(s) in relation to the problem that has to be solved;
- analyse spectra to obtain physical and chemical properties of molecules;
- interpret spectroscopic results in terms of underlying structure and dynamics.


Lectures, tutorials, practical’s and lab projects, reporting.


The course will be concluded with an open-book written exam with open questions. The minimum mark for a pass is 5.5. The final mark can be assessed only after completing all practical courses. Partial results remain valid for 5 years.


Atkins, P.; de Paula, J. (2017). Atkins' Physical Chemistry, 11th ed.
Reader Spectroscopy and Imaging.

Compulsory for: BMLMolecular Life SciencesBSc5AF