ORC-11806 Analytical Methods in Organic Chemistry


Credits 6.00

Teaching methodContact hours
Course coordinator(s)dr. TA van Beek
Lecturer(s)dr. TA van Beek
prof. dr. MWF Nielen
dr. ir. HM Willemen
dr. ir. LCPM de Smet
Examiner(s)dr. TA van Beek
dr. ir. LCPM de Smet
prof. dr. MWF Nielen

Language of instruction:

English and Dutch

Assumed knowledge on:

ORC-12803 Organic Chemistry 1 and ORC-12903 Organic Chemistry 2.


This course introduces the student:
(1) to both theory and practical application of chromatographic analyses and separation techniques, and understanding and using basic isolation techniques for components of various chemical classes. Attention is given to the qualitative and quantitative analysis of, e.g., preservatives, drugs, sugars and pollutants using a variety of techniques (e.g. TLC, HPLC, GC, capillary electrophoresis (CE) and ELISA.
(2) to both theory and practical applications of spectroscopic methods aiming towards the structural analysis of organic compounds, e.g., as obtained via the methods described under
(1). The combination of UV/VIS, IR & NMR spectroscopy and mass spectrometry (MS) is discussed and used. During the practical work these methods will be applied towards the elucidation of the structure of unknown compounds.
(3) special emphasis is also given to combining (1) and (2): the application of GC-MS and LC-MS including theory, scope and limitations, and practical usage in the analysis of food contaminants.

Learning outcomes:

After successful completion of this course students are expected to be able to:
- understand chromatographic and spectroscopic theory and use this to solve problems;
- apply various isolation and chromatographic techniques to separate compounds;
- appraise published analytical procedures and explain the logic behind them;
- apply spectroscopic and sample preparation techniques to obtain spectra from compounds;
- interpret recorded spectra and propose a chemical structure based on those data;
- prepare a written report that summarises collected data in a clear fashion.


- attend lectures;
- exercises classes and case study;
- study of the book and reader;
- doing practical experiments and simulation experiments;
- report writing.


- the final mark is determined by the written exam (60%);
- the practicals and reports chromatography (40%);
The minimum mark for each part is 5.00. The minimum average mark of the 2 parts to pass the course is 5.50. The written exam has open questions. Mark for practical work is based on practical level and input during practical classes and reports. The marks for the individual parts of this course will remain valid for 4 academic years.


McMurry, J. (2012). Organic Chemistry. 8th International editition. Pacific Grove, CA [etc.], US: Brooks/Cole. 1262p. ISBN 084005453X.
Reader: ORC-11806 Analytical Methods in Organic Chemistry.

Compulsory for: BBTBiotechnologyBSc6WD
BMLMolecular Life SciencesBSc1AF
Restricted Optional for: BLSBachelor Orientation year Life SciencesBSc6WD
Restricted Optional for: WUCHMBSc Minor Chemical Sciences1AF